|
KMID : 0613820190290010060
|
|
Journal of Life Science
2019 Volume.29 No. 1 p.60 ~ p.68
|
|
|
Antiadipogenic Activity of Solvent-partitioned Fractions from Limonium tetragonum in 3T3-L1 Preadipocytes
|
|
Kwon Myeong-Sook
Kim Jung-Ae
Oh Jung-Hwan
Karadeniz Fatih
Lee Jung-Im
Seo Young-Wan
Kong Chang-suk
|
|
|
Abstract
|
|
|
|
Limonium tetragonum, an edible halophyte that grows on salt marshes in Korea, is thought to possess various health benefits (e.g., antioxidant, antitumor, and hepatoprotective). In the present study, different solvent partitioned subfractions, water (H2O), buthanol (n-BuOH), 85% aqueous methanol (85% aq. MeOH), and hexane (n-hexane), from crude extract of L. tetragonum were tested for their ability to prevent adipogenesis in differentiating 3T3-L1 preadipocytes. The treatment of differentiating 3T3-L1 preadipocytes with L. tetragonum subfractions (LTFs) resulted in suppressed adipogenesis and reduced expression of adipogenesis-related transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ), CCAATT/enhancer-binding protein alpha (C/EBPα), and sterol regulatory element-binding protein 1c (SREBP-1c) at both mRNA and protein levels. In addition, the LTF treatment notably decreased the levels of phosphorylated p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) of the mitogen-activated protein kinase (MAPK) pathway in association with PPARγ-linked adipogenesis. Among all the tested LTFs, H2O and n-hexane were the most effective in lowering lipid accumulation and regulating the adipocyte differentiation via PPARγ pathway. Taken together, the results indicated that the H2O and n-hexane LTFs contain bioactive compounds that may exhibit significant antiadipogenesis activity by downregulation of the PPARγ pathway and inactivation of the MAPK signal pathway in 3T3-L1 preadipocytes.
|
|
|
KEYWORD
|
|
|
3T3-L1, Adipogenesis, Limonium tetragonum, MAPK, PPARγ
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|